normal human lung fibroblasts mrc 5  (ATCC)

Journal: Cell Communication and Signaling : CCS

Article Title: Annexin A2-dependent extracellular vesicle proteome modulates pre-metastatic stromal fibroblast behavior in triple-negative breast cancer

doi: 10.1186/s12964-026-02708-3

Figure Lengend Snippet: EVs derived from AnxA2-expressing cells elicit distinct functional and activation-associated responses in MRC-5 fibroblasts. A Representative confocal images of MRC-5 fibroblasts treated for 4 h with PKH26-labeled EVs from NC or KD2 LM2 cells. DAPI (blue), PKH26 (red), and merged channels are shown; scale bar, 20 μm. B Quantification of normalized PKH26 fluorescence intensity (n = 3). C Representative scratch wound images at 0 h and 24 h; scale bar, 100 μm. D Quantification of wound closure at 24 h (n = 3). E Western blot analysis of α-SMA and FAP expression in MRC-5 cells treated with PBS, NC-EVs or KD2-EVs. F Quantification of protein expression relative to GAPDH and normalized to the TGF-β1 treated group (n = 3). The data represent the mean ± SEM of independent biological replicates. Statistical comparisons were performed using an unpaired two-tailed t-test with Welch’s correction ( B ) or one-way ANOVA with Tukey’s multiple-comparisons test ( D ). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant

Article Snippet: Normal human lung fibroblast lines MRC-5 (#CCL-171) and WI-38 (#CCL-75) were obtained from the American Type Culture Collection (ATCC).

Techniques: Derivative Assay, Expressing, Functional Assay, Activation Assay, Labeling, Fluorescence, Western Blot, Two Tailed Test

Journal: Microbial Cell Factories

Article Title: Recombinant L-asparaginase from Stenotrophomonas maltophilia : a promising low-immunogenic anticancer agent

doi: 10.1186/s12934-025-02856-0

Figure Lengend Snippet: Cytotoxic activity of recombinant L-asparaginase produced by E. coli BL21 (DE3)-pET22b(+)-ASP and the marketed E. coli L-asparaginase on MRC-5 cells. The results denote the average of the data received from three experiments and the error bars show standard deviation

Article Snippet: Human leukemia cancer cell line K-562, human hepatocellular carcinoma cell line HepG-2, and normal human lung fibroblast cell line MRC-5 were purchased from the American Type Culture Collection (ATCC, Rockville, MD).

Techniques: Activity Assay, Recombinant, Produced, Standard Deviation